product summary
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company name :
MyBioSource
product type :
protein
product name :
Phosphodiesterase 1
catalog :
MBS653995
quantity :
100 Units
price :
285 USD
more info or order :
product information
catalog number :
MBS653995
products type :
Enzyme
products full name :
Phosphodiesterase 1
products short name :
Phosphodiesterase 1
other names :
phosphodiesterase 1; phosphodiesterase 1
other gene names :
PDE1
host :
Crotalus adamanteus venom (Eastern Diamondback rattlesnake)
purity :
Molecular Biology Grade
form :
Supplied as a lyophilized powder
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
other info1 :
Activity: 130 u/mg dry weight, 110 u/vial. Absorbance (A280): 0.82. Nucleotidase: 0.80%. Optimum pH: 9.8-10.4. Inhibitors: Reducing agents such as glutathione, cysteine and ascorbic acid. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. Activators: The enzyme has an absolute requirement for Mg2+ indicates an optimum concentration of 15mM.
other info2 :
Unit Definition: 1 unit hydrolyzes 1 umole of p-nitrophenyl thymidine-5-phosphate per minute at 25 C, pH 8.9.
products categories :
Molecular Biology; MB-Enzymes, Phosphodiesterase
products description :
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-and deoxyribo-oligonucleotides. The enzyme has been widely utilized as a tool for structural and sequence studies of nucleic acids. The enzyme has been purified with endonuclease activity being eliminated as well as 5'-nucleotidase and nonspecific monophosphatase. It is nonspecific with respect to base or sugar moieties of nucleotides. A variety of synthetic substrates are hydrolyzed. The exonuclease will not recognize nucleoside units in the syn conformation. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP.
ncbi gi num :
148539632
ncbi acc num :
NP_001091918.1
ncbi gb acc num :
NM_001098448.1
ncbi mol weight :
115kD
ncbi pathways :
Purine Metabolism Pathway (85597); Purine Metabolism Pathway (307)
size1 :
100 Units
price1 :
285 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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