protein

Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl-beta-glucosaminidase)

MBS653950

25 Units

675 USD

Enzyme

Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl-beta-glucosaminidase)

[Chitinase, Trichoderma viride]

[Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl- -glucosaminidase)]

[chitinase]

Molecular Biology Grade

Supplied as a tan lyophilized powder.

Lyophilized powder may be stored at 4 degree C for short-term only. Stable at -20 degree C for 6 months after receipt. Reconstitute with sterile PBS. Store at -20 degree C. Reconstituted product is stable for 5 weeks at 4 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Structure

Source: Trichoderma viride. Activity: Chitinase Activity:(>/=600 units/g solid) 1183u/g. Chitobiosidase Activity: 169u/mg. Nagase Activity: 1768u/mg. Chitinase Unit Definition: One unit will liberate 1mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 degree C in a 2 hour assay. Chitobiosidase Unit Definition: N-Acetyl-b-glucosaminidase. [Chitobiosidase]: . One unit will release 1.0 mg of 4-nitrophenol from 4-nitrophenyl N-Acetyl-b-Dglucosaminide [4-nitrophenyl N,N'-diacetyl -b--Dchitobioside] per minute at pH 6.1 at 40 degree C. Assay: Reaction mixture is composed of 30mg of N-acetyl chitosan, 2ml of 0.1M Mcllvaine buffer (pH 6.8) and 1ml of enzyme solution. After incubation at 37°C for 60 minutes, the reaction of the enzyme is terminated by heating at 100 degree C for 5 minutes and the amount of reducing sugar is determined by the method of Schales and Schales, using N-acetyl glucosamine as a standard.

Solubility: Soluble in phosphate buffer, pH 6.0, (1mg/ml), yielding a clear to faint hazy, tan solution

Molecular Biology; MB-Enzymes, Glycosidase

The chitinolytic enzymes from T. viride are a mixture of extracellular chitinolytic enzymes, which exhibit exo and endochitinase activities including N-acetyl- -glucosaminidase and chitobiosidase. Chitinases are digestive enzymes that break down glycosidic bonds in chitin. Because chitin composes the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), chitinases are generally found in organisms that either need to reshape their own chitin or to dissolve and digest the chitin of fungi or animals.

149688626

ABR27743.1

110-115kD

25 Units

675 USD