protein

Trypsin 1:250 (Porcine)

MBS653921

25 g

170 USD

Enzyme

Trypsin 1:250 (Porcine)

[Trypsin 1:250]

[Transcription intermediary factor 1-beta; Transcription intermediary factor 1-beta; transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; tripartite motif containing 28; E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28]

[TRIM28; TRIM28; KAP1; TF1B; RNF96; TIF1B; KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1]

TIF1B_HUMAN

Porcine pancreas

Cell Culture Grade. Chromatographically-purified. SDS-PAGE. Certified to be free of porcine parvovirus.

Off-white to beige crystalline powder

Powder may be stored at 4 degree C. Stable for 12 months at 4 degree C.

CAS Number: 9002-07-7. Country of Origin: Canada

Trypsin: 307u/mg. Chymotrypsin: 45u/mg. Endotoxin: <0.5EU/mg. Ash: <0.1%. Moisture: 4.6%. Microbiological Analysis: Porcine Parvovirus (Hemagglutination, PCR): None Detected. Plate Count: <1000cfu/g. Yeast/Mold: <10cfu/g. P. aeruginosa: None Detected. Salmonella: None Detected. E. coli: None Detected. S. aureus: <10cfu/g. Unit Definitions: Trypsin: The amount of enzyme which causes an increase in absorbance at 253nm of 0.003 per minute at 25 degree C Chymotrypsin: The amount of enzyme which causes an decrease in absorbance at 237nm of 0.0075 per minute at 25 degree C.

Molecular Biology; MB-Enzymes

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation.

3183179

Q13263.5

Q13263

C-MYB Transcription Factor Network Pathway (138073); E2F Transcription Factor Network Pathway (137934); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); P53 Pathway (138067)

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Function: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Ref.1 Ref.2 Ref.10 Ref.13 Ref.14 Ref.15 Ref.18 Ref.21 Ref.23 Ref.24 Ref.25 Ref.27 Ref.29 Ref.38 Ref.39 Ref.42 Ref.44. Pathway: Protein modification; protein sumoylation. Subunit structure: Oligomer; the RBCC domain homotrimerizes and interacts with one molecule of KRAB to form the KRAB-KAP1 corepressor complex. Binding to a KRAB domain is an absolute requirement for silencing gene expression. Interacts with CEBPB and NR3C1 . By similarity. Interacts with a number of KRAB-ZFP proteins including ZNF10, ZFP53, ZFP68, ZNF382 and ZNF256. Interacts with NCOR1, NR3C1 and CHD3. Interacts with CEBPB (via the RING-type and PHD-type zinc fingers). Component of a ternary complex that includes TRIM28, a HP1 protein (CBX1, CBX3 OR CBX5), a KRAB domain-containing protein, and DNA. Interacts with CBX5 (via the PxVxL motif); the interaction occurs in interphase nuclei and competes for binding POGZ. Interacts with POGZ; the interaction competes for interaction with CBX5. Interacts with SETDB1; the interaction is enhanced by KAP1 sumoylation, stimulates SETB1 histone methyltransferase activity and gene silencing. Interacts (via the PHD-type zinc finger) with UBE2I; the interaction is required for sumoylation and repressor activity. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor and DNA damage responses. Interacts directly with ERBB4; the interaction represses ERBB4-mediated transcription activity. Interacts with MDM2; the interaction contributes to p53/TP53 inactivation. Component of the TRIM28/KAP1-MDM2-p53/TP53; involved in regulating p53/TP53 stabilization and activity. Interacts (via the leucine zipper alpha helical coiled-coil) with E2F1 (central region); the interaction inhibits E2F1 acetylation and transcriptional activity. Interacts with PPP1CA; the interaction dephosphorylates TRIM28 at Ser-824 and forms a complex at the p21 promoter site. Interacts with PPP1CB; the interaction is weak but is increased on dephosphorylation at Ser-824. Interacts with FES/FPS. Interacts with SMARCAD1. Interacts with, and sumoylates IRF7. Interacts with MAGEC2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with AICDA . By similarity. Ref.2 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.22 Ref.23 Ref.24 Ref.27 Ref.29 Ref.30 Ref.38 Ref.39 Ref.40 Ref.42 Ref.44 Ref.45. Subcellular location: Nucleus. Note: Associated with centromeric heterochromatin during cell differentiation through CBX1 . By similarity. Ref.2 Ref.9 Ref.18 Ref.38. Tissue specificity: Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Ref.2. Domain: The HP1 box is both necessary and sufficient for HP1 binding. Ref.8 Ref.27The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain. Ref.8 Ref.27The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization. Ref.8 Ref.27Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. Ref.8 Ref.27. Post-translational modification: ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. Ref.23 Ref.25 Ref.27 Ref.37 Ref.42 Ref.48Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. Ref.23 Ref.25 Ref.27 Ref.37 Ref.42 Ref.48Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2. Sequence similarities: Belongs to the TRIM/RBCC family.Contains 2 B box-type zinc fingers.Contains 1 bromo domain.Contains 1 PHD-type zinc finger.Contains 1 RING-type zinc finger.

25 g

170 USD

100 g

310

500 g

570

1 kg

960