catalog number :
MBS653874
products full name :
Phosphatase, Alkaline, Intestine, Bovine (Alkaline Phosphatase Intestinal, ALPI, Alkaline Phosphomonoesterase, Glycerophosphatase, Intestinal Alkaline Phosphatase, IAP, Intestinal Alkaline Phosphatase V, IAP-V, Kasahara Isozyme)
products short name :
[Phosphatase, Alkaline, Intestine, Bovine]
form :
Supplied as liquid, crystalline suspension in 70% ammonium sulfate. Free of endonuclease, exonuclease and RNase activities. No preservatives added.
concentration :
27.44mg/ml
storage stability :
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA). Other applications not tested
app notes :
May be diluted in sterile ddH2O or in dilute buffer. Optimal dilutions to be determined by the researcher.
other info1 :
. Source: Calf intestine . Activity: 4000U/mg protein. 1 unit liberates 1umole of p-nitrophenol per minute at 37°C and pH 9.8 in DEA buffer. Activity is determined by measuringthe increase in the optical density at 405nm resulting from the hydrolysis of p-notrophenyl phosphate.
products categories :
Molecular Biology; MB-Enzymes, Phosphatase
products description :
Native calf alkaline phosphatase.
ncbi mol weight :
110-150kD