protein

GPX4 (Glutathione Peroxidase 4, Phospholipid Hydroperoxidase, HGNC:4556, PHGPx, snGPx, sperm nucleus glutathione peroxidase) (Control Peptide)

MBS653861

0.1 mg

400 USD

Enzyme

GPX4 (Glutathione Peroxidase 4, Phospholipid Hydroperoxidase, HGNC:4556, PHGPx, snGPx, sperm nucleus glutathione peroxidase) (Control Peptide)

[GPX4]

[GPX4 protein; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase; glutathione peroxidase 4; Glutathione peroxidase 4]

[GPX4; GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx; PHGPx; GPx-4; GSHPx-4]

GPX4_HUMAN

C-terminus of aa-EEPLVIEKDLPHY

Recognizes human GPX4.

Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile ddH2O

~0.5 mg/ml (after reconstitution)

Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA) and Antibody blocking

Optimal dilutions to be determined by the researcher

Testing Data

Testing Data

Source: Synthetic.

Molecular Biology; MB-Enzymes, Peroxidase

C-terminus of aa-EEPLVIEKDLPHY. Control Peptide corresponding to polyclonal antibody, MBS623380, goat x human. Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

93214392

AAH32695.3

P36969

22,175 Da

Arachidonic Acid Metabolism Pathway (685553); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Synthesis Of 12-eicosatetraenoic Acid Derivatives Pathway (730337)

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008]

Function: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage . By similarity. Catalytic activity: 2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O. Subunit structure: Monomer. Has a tendency to form higher mass oligomers. Ref.7. Subcellular location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Tissue specificity: Present primarily in testis. Sequence similarities: Belongs to the glutathione peroxidase family.

0.1 mg

400 USD