protein

Aromatic L-Amino Acid Decarboxylase, Human (DOPA Decarboxylase, DDC) (Control Peptide)

MBS653838

0.05 mg

240 USD

Enzyme

Aromatic L-Amino Acid Decarboxylase, Human (DOPA Decarboxylase, DDC) (Control Peptide)

Aromatic L-Amino Acid Decarboxylase

aromatic L-amino acid decarboxylase; Aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase

DDC; DDC; AADC; AADC; AADC; DDC

DDC_HUMAN

Human

A 16aa peptide sequence newar the N-terminus of human DDC.

16aa peptide shows homology with rat, mouse and pig (93%); bovine and guinea pig (87%).

Highly Purified. Highly purified

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

~1mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA)

ELISA: Use at 1ug/ml to coat plates and detect with antibodies (1;10,000-1:50,000)., Note: Not suitable for Western Blot due to low MW (< 3kD).

Molecular Biology; MB-Enzymes, Decarboxylase

DDC (EC 1.1.128) belongs to the family group II Decarboxylase (DDC, GAD, HDC and TYRD). DDC catalyzes the formation of tryptamine from L-tyrosine. It also acts on 5-hydroxy-LO-tryptophan and dihydroxy-L-Phenylalanine (DOPA). Human DDC (480aa, chromosome 7p11) is a homodimer in the native state. it requires pyridoxal phosphate as a cofactor. A 16 amino acid peptide sequence near the N-terminus of human DDC.

257538

AAB23675.1

P20711

53,926 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Cocaine Addiction Pathway (546258)

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Catalytic activity: L-dopa = dopamine + CO2.5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO2. Cofactor: Pyridoxal phosphate. Ref.10. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. Subunit structure: Homodimer. Ref.10. Involvement in disease: Aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]: An inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.11 Ref.14 Ref.15. Sequence similarities: Belongs to the group II decarboxylase family.

0.05 mg

240 USD