protein

DGAT2 (Diacylglycerol O-acyltransferase homolog 2, DGAT2, DKFZp686A15125, HMFN1045, GS1999full) (Control Peptide)

MBS653702

0.1 mg

400 USD

Blocking Peptide

DGAT2 (Diacylglycerol O-acyltransferase homolog 2, DGAT2, DKFZp686A15125, HMFN1045, GS1999full) (Control Peptide)

[DGAT2]

[diacylglycerol O-acyltransferase 2 isoform 2; Diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase 2; diglyceride acyltransferase 2; retinol O-fatty-acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; diacylglycerol O-acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2]

[DGAT2; DGAT2; ARAT; HMFN1045; GS1999FULL; ARAT; Retinol O-fatty-acyltransferase]

DGAT2_HUMAN

Highly Purified

Supplied as a lyophilized powder from ddH20, 5% (v/v) acetonitrile. Reconstitute With 200ul sterile ddH20.

Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. Reconstite with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Anitbody Blocking

Suitable for use in ELISA. Optimal dilutions to be determined by the researcher.

Testing Data

Testing Data

Source: Synthetic. Grade: Highly Purified

Peptide Blocking (see Form: corresponding antibody MBS622807): Antibodies are typically supplied at O.5mg/ml and peptides as a 100ug pellet. When peptldes are reconstituted pp Ica Ions: In 200ul water, the concentration would be also O.5mg/ml. To start, the best ratio Recommended Dilution would be 1:1 (which means molar excess of peptldes relative to antibodies when identical volumes are mixed), Mix equal volumes of peptide and antibody Storage and Stability: at the required dilution and leave at ambient temp'erature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Molecular Biology; MB-Enzymes, Acyltransferase

C-terminus of aa-KLEHPTQQDIDLYH. Control peptide corresponaing to polyclonal antibody

359806523

NP_001240820.1

NM_001253891.1

Q96PD7

Acyl Chain Remodeling Of DAG And TAG Pathway (645315); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycerophospholipid Biosynthesis Pathway (645313); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Phospholipid Metabolism Pathway (645312)

This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Function: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides . By similarity. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Catalytic activity: Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.9Acyl-CoA + retinol = CoA + retinyl ester. Ref.9. Enzyme regulation: Inhibited by niacin . By similarity. Pathway: Glycerolipid metabolism; triacylglycerol biosynthesis. Subunit structure: Forms multimeric complexes consisting of several DGAT2 subunits . By similarity. Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet . By similarity Ref.2. Tissue specificity: Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin. Ref.1 Ref.2. Sequence similarities: Belongs to the diacylglycerol acyltransferase family. Sequence caution: The sequence BAD38635.1 differs from that shown. Reason: Erroneous initiation. The sequence CAD38961.1 differs from that shown. Reason: Erroneous initiation.

0.1 mg

400 USD