protein

Zymolyase 100T (Lyticase, Yeast Lytic Enzyme)

MBS653685

25 mg

195 USD

Enzyme

Zymolyase 100T (Lyticase, Yeast Lytic Enzyme)

Zymolyase 100T

Arthrobacter luteus

Molecular Biology Grade. Purified by affinity chromotography.

Lyophilized powder

Stable for 1 year at 4 degree C. About 90% of the lytic activity is lost when stored at 30 degree C for 3 months.

Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation

CAS Number: 37340-57-1. Activity: 105 u/mg. Contaminants: DNase, Rnase: Trace levels detected. Essential Enzyme: beta-1 ,3-glucan laminaripentaohydrolase. beta-l,3-glucanase: 1.0xl0e7 units/g. Protease: 1.7xl0e4 units/g. Mannanase: 6.0x 1 Oe4 units/g. Optimum pH: 7.5@35 degree C, For lysis of viable yeast cells. 6.5@45 degree C, For hydrolysis of yeast glucan. Stable pH: 5-10.

Storage Buffer: 0.06M Phosprate Buffer, pH 7.5Note: Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension. Unit Definition: One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer to Assay for Enzymatic Activity. Heat Stability: Lytic activity lost on incubation at 60 degree C for 5 min. Activators: SH compounds - 2-mercaptoethanol, cysteine or dithiothreitol. RNase Activity Assay: Trace RNase activity was detected. Method: . MBS653685 was suspended in 50mM Tris-HCI bufferhPH 7.5. 10ul of MBS653685 was mixed wit 140ul of cCMP (0.1 mg/ml in 50mM Tris-HCI buffer, pH 7.5) and the increase of absorbance at 284nm was measured. Unit Definition: . One RNase A unit is defined as the amount required for hydrolysis of cCMP that results in an increase of 0.001 absorbance units at 284nm per minute. DNase Activity Assay: Trace DNase activity was detected. Method:. MBS653685 was suspended in 50mM Tris-HCI buffer, pH 7.5, at a concentration of 2mg/ml and centrifuged to remove the debris. The supernantant was used for the DNase assay. Reaction mixture (50mM Tris-HCI, pH 7.5, 1 mM MnCI2, 50ng/ul lambda DNA-Hindill-digest, 0.4mg/ml MBS653685) was incubated at 30 degree C for 12 hours, Inactivated by heating, and the degradation of lambda DNA was detected by using agarose gel electrophoresis. Experimental Precautions: 1. Avoid nitrocellulose filters due to non-specific adsorption onto the membrane. 2. If using sterilized MBS653685 in concentrations higher than 0.05%, prepare a 2% solution in buffers containing 5% glucose. Filter the suspension and then dilute with an appropriate buffer.

Molecular Biology; MB-Enzymes

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below.

25 mg

195 USD

50 mg

285

100 mg

405

250 mg

670