product summary
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company name :
MyBioSource
product type :
protein
product name :
Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
catalog :
MBS653642
quantity :
1 mL
price :
155 USD
more info or order :
product information
catalog number :
MBS653642
products type :
Enzyme
products full name :
Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
products short name :
Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol
purity :
Molecular Biology Grade
form :
Clear to pale yellow solution/suspension. Activity: 100u/mg. Purity: Purified by affinity chromotography. Unit Definition:. One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer
storage stability :
Stable for 12 months at -20 degree C.
app notes :
Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation
other info1 :
CAS Number: 37340-57-1. Water: Molecular Biology Grade, RNase, DNase-Free. Filter: 0.2um validated. Sorbitol: 98%
other info2 :
Note: Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension.
products categories :
Molecular Biology; MB-Enzymes
products description :
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Zymolyase 100T Stock Concentrate 10mg/ml is a convenient preparation for use in yeast spheroplast/protoplast fusion and transformation protocols. Also useful for yeast tetrad dissection solutions.
size1 :
1 mL
price1 :
155 USD
size2 :
5 mL
price2 :
300
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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