catalog number :
MBS653421
products type :
Culture Media
products full name :
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red (Powder)
products short name :
[RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red]
specificity :
RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et al. at Roswell Park Memorial Institute.
purity :
Cell Culture Grade
storage stability :
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time
other info1 :
Appearance: White, homogeneous, free flowing powder. Solubility: Colorless, clear, complete. pH: 8.3. Endotoxin: < = 1 EU/ml
other info2 :
Directions per Liter: Dissolve 7.4g in 800-900mL of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medioum to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 um membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Inorganic Salts: Calcium Nitrate•4H2O 0.1. Magnesium Sulfate 0.04884. Potassium Chloride 0.4. Sodium Chloride 6. Sodium Phosphate Dibasic 0.8. Amino Acids: L-Arginine Absent. L-Aspartic Acid Absent. L-Asparagine Absent. L-Cystine•2HCl Absent. L-Glutamic Acid Absent. L-Glutamine Absent. Glycine Absent. L-Histidine Absent. Hydroxy-L-proline Absent. L-Isoleucine Absent. L-Leucine Absent. L-Lysine•HCl Absent. L-Methionine Absent. L-Phenylalanine Absent. L-Proline Absent. L-Serine Absent. L-Threonine Absent. L-Tryptophan Absent. L-Tyrosine•2Na•2H2O Absent. L-Valine Absent. Vitamins: P-Amino Benzoic Acid 0.001. D-Biotin 0.0002. Choline Chloride 0.003. Vitamin B-12 0.000005. Folic Acid 0.001. myo-Inositol 0.035. Niacinamide 0.001. D-Pantothenic Acid•Ca 0.00025. Pyridoxine•HCl 0.001. Riboflavin 0.0002. Thiamine•HCl 0.001. Other: Glucose Absent. Glutathione 0.001. Phenol Red, Sodium Absent. Total: 7.4g/liter
products categories :
Culture Media; Media-Cell Culture, RPMI
products description :
RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI.