catalog number :
MBS653087
products type :
Culture Media
products full name :
Dulbecco's MEM (DMEM) Low Glucose, w/o Amino Acids, Pyruvic Acid (Powder)
products short name :
[Dulbecco's MEM]
purity :
Cell Culture Grade
storage stability :
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.
other info1 :
Appearance: Light orange, homogenous, free-flowing powder. Solubility: Yellow, clear, complete. pH: 4.8. Endotoxin Level: < or = 1 EU/ml
other info2 :
Directions per Liter: Dissolve 8.32g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Components shown as: g/Liter. Inorganic Salts: Calcium Chloride•2H2O: 0.265. Ferric Nitrate•9H2O: 0.0001. Magnesium Sulfate: 0.09767. Potassium Chloride: 0.4. Sodium Chloride: 6.4. Sodium Phosphate Monobasic: 0.109. Amino Acids: L-Arginine•HCl- Absent. L-Cystine•2HCl- Absent. L-Glutamine- Absent. Glycine- Absent. L-Histidine•HCl•H2O- Absent. L-Isoleucine- Absent. L-Leucine- Absent. L-Lysine•HCl- Absent. L-Methionine- Absent. L-Phenylalanine- Absent. L-Serine- Absent. L-Threonine- Absent. L-Tryptophan- Absent. L-Tyrosine•2Na•2H2O- Absent. L-Valine- Absent. Vitamins: Choline Chloride: 0.004. Folic Acid: 0.004. myo-Inositol: 0.0072. Niacinamide: 0.004. D-Pantothenic Acid•Ca: 0.004. Pyridoxal•HCl: 0.004. Riboflavin: 0.0004. Thiamine•HCl: 0.004. Other: D-Glucose: 1. Phenol Red, Sodium: 0.0159.
Pyruvic Acid, Sodium- Absent. Total: 8.32g/Liter.
products categories :
Culture Media; Media-Cell Culture, DMEM
products description :
Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.