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RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Powder)

MBS653004

10 L

195 USD

Culture Media

RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Powder)

[RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin]

Cell Culture Grade

Light pink, homogenous, free flowing powder

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Endotoxin Level: <=1EU/ml

pH: 8.6. Solubility: Pink. clear, complete. Directions per Liter: Dissolve 10.092g in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2.0g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate. Media Formulation: Components shown as g/L . Inorganic Salts . Calcium Nitrate*4H2O: 0.100. Magnesium Sulfate: 0.04884. Potassium Chloride: 0.400. Sodium Chloride: 6.000. Sodium Phosphate Dibasic: 0.800. Amino: Acids . L-Alanine: Absent. L-Arginine: 0.200. L-Asparagine: 0.050. L-Aspartic Acid: 0.020. L-Cystine*2HCl: 0.065. L-Glutamic Acid: 0.020. L-Glutamine: Absent. Glycine: 0.010. L-Histidine: 0.015. Hydroxy-L-proline: 0.020. L-Isoleucine: 0.050. L-Leucine: 0.050. L-Lysine*HCl: 0.040. L-Methionine: 0.015. L-Phenylalanine: 0.015. L-Proline: 0.020. L-Serine: 0.030. L-Threonine: 0.020. L-Tryptophan: 0.005. L-Tyrosine*HCl: 0.028. L-Valine: 0.020. Vitamins . p-Aminobenzoic Acid: 0.001. D-Biotin: 0.0002. Choline Chloride: 0.003. Cyanocobalamin (Vit.B12): 0.000005. Folic Acid: Absent. myo-Inositol: 0.035. Niacinamide: 0.001. D-Pantothenic Acid, Ca: 0.00025. Pyridoxal*HCl: 0.001. Riboflavin: Absent. Thiamine*HCl: 0.001. Other . D-Glucose: 2.000. Glutathione, reduced: 0.001. Phenol Red, Sodium: 0.0053. Total: 10.092g/L

Culture Media; Media-Cell Culture, RPMI

RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

10 L

195 USD

25 L

350

50 L

570

100 L

830