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MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red (Powder)

MBS652906

25 L

235 USD

Culture Media

MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red (Powder)

MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red

Cell Culture Grade

White to light yellow, free flowing, homogeneous powder.

Store podered media at Room Temperature. Opended bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Solubility: Colorless to yellow, clear, complete. pH: 6.69. Endotoxin: < or = 1 EU/ml

Directions per Liter: Dissolve 9.27g in 800-900ml of ddH20, stirring gently until completely solubilized. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1 L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into steriie containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Analysis: POWDER . Components: . InorganicSalts=mg/liter. Calcium Chloride•2H20 = 264.90. Magnesium Sulfate = 97.70. Potassium Chloride = 400.00. Sodium Chloride = 6800.00. Sodium Prosphate. Monobasic•H20 = 140.30. Amino Acids: . L-Arginine•HCI = 126. 40 L-Cystine24.= 02. L-Glutamine = Absent. L-Histidine•HCI•H20 = . 42 L-Isoleucine = 52. L-Leucine = 52. L-Lysine•HCI = 72.5. L-Methionine = 15. L-Phenylalanine = 32. L-Threonine = 48. L-Tryptophan = 10. L-Tyrosine = 36. L-Valine = 46. Vitamins: . Choline Chloride = l.00. Folic Acid = Absent. i-Inositol = 2.00. Nicotinamide = l.00. D-Pantothenic Acid•Ca = l.00. Pyridoxal•HCI = l.00. Riboflavin0.= 10. Thiamine•HCI = 1.00. Other: . D-Glucose = 1000.00. Phenol Red, Sodium =Absent. Total: 9.266g/L.

Culture Media; Media-Cell Culture, MEM Eagle

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.

25 L

235 USD

50 L

300

100 L

430