catalog number :
MBS652861
products type :
Culture Media
products full name :
Brain Heart Infusion Broth w/o Dextrose (Powder)
products short name :
Brain Heart Infusion Broth w/o Dextrose
purity :
Microbiological Grade
storage stability :
Store powdered media at RT/4 degree C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
other info1 :
pH: 7.35. Solubility: Light to medium amber, clear and complete. Appearance: Beige homogeneous, free flowing powder
other info2 :
Direction per Liter: Dissolve in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers. Loosen caps and autoclave for 15 minutes at 121 degree C (15psi). Components: Components shown as: g/liter . Brain Heart Infusion from Solids: 17.5 g/liters. Disodium Phosphate: 2.5 g/liters. Pancreatic Digest of Gelatin: 10.0 g/liters. Sodium Chloride: 5.0 g/liters. Total: 35 g/liters
products categories :
Culture Media; Media-Microbiological
products description :
Cultivation of fastidious aerobic and anaerobic bacteria. Brain Heart Infusion Broth without Dextrose is a modification of the media described by Rosenow and Hayden in which infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer. The addition of 0.1% agar can be used to lower oxygen tension to enhance the growth of anaerobic organisms. Brain Heart Infusion Broth without Dextrose is a basal medium used with added carbohydrates for fermentation studies.