catalog number :
MBS652776
products type :
Culture Media
products full name :
MCDB 110 Diploid Fibroblast-like Cell Medium (Powder)
products short name :
[MCDB 110 Diploid Fibroblast-like Cell Medium]
purity :
Cell Culture Grade
form :
White to light orange, homogeneous, free flowing powder
storage stability :
Store powdered media at RT. Opened bottles should becapped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
other info1 :
Appearance: White, homogeneous, free flowing powder. Endotoxin Level: 1EU/ml. Solubility (15.3 g/L): Pale yellow, clear, complete. pH: 6.5
. Directions per Liter: Dissolve 15.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Add sodium bicarbonate if required.Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Components shown as mg/liters: Inorganic Salts: . Ammonium Metavandate: 0.000585. Ammonium Molybdate: 0.00124. Calcium Chloride 2H20: 147.00. Cupric Sulfate 5H20: 0.00025. Ferrous Sulfate 7H20: 1.39. Magnesium Sulfate: 120.38. Manganese Sulfate: 0.000151. Nickel Chloride Hexahydrate: 0.00012. Potassium Chloride: 372.75. Sodium Chloride: 6546.00. Sodium Metasilicate 9H2O: 0.1421. Sodium Phosphate Dibasic: 425.94. Sodium Selenite: 0.0038. Stannous Chloride Dihydrate: 0.000113. Zinc Sulfate 7H2O : 0.144. Amino Acids: . L-Alanine: 8.90 . L-Arginine HCl: 210.70. L-Asparagine H2O: 15.00. L-Aspartic Acid: 13.31. L-Cysteine HCl: H2O 8.78. L-Glutamic Acid: 14.71. L-Glutamine: 365.30. Glycine: 22.52. L-Histidine HCl: H2O 20.97. L-Isoleucine: 3.94. L-Leucine: 13.12. L-Lysine HCl: 36.54. L-Methionine: 4.48. L-Phenylalanine: 4.96. L-Proline: 34.53. L-Serine: 10.51. L-Threonine: 11.91. L-Tryptophan: 2.04. L-Tyrosine: 7.84. L-Valine: 11.72. Vitamins: . Biotin: 0.00733. Choline Chloride: 13.96. Vitamin B12: 0.136. Folinic Acid, Calcium: 0.000602. Myo-Inositol: 18.02. Nicotinamide: 6.11. D-Pantothenic Acid Ca: 0.2383. Pyridoxine HCl: 0.0617. Riboflavin: 0.113. Thiamine HCl: 0.337. Other: . Adenine HCl: 1.72. Thymidine: 0.0727. D-Glucose: 720.64. HEPES Free Acid: 5958.00. Thioctic Acid: 0.00206. Phenol Red, Sodium: 1.242. Putrescine 2HCl: 0.0001611. Sodium Pyruvate: 110.00. Total: 15.3g/liter
products categories :
Culture Media; Media-Cell Culture, MCDB
products description :
Basal Medium MCDB 110 is specifically formulated for the cultivation of diploid fibroblast-like cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).