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Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)

MBS652750

25 L

285 USD

Culture Media

Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)

[Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate]

Cell Culture Grade

Light pink, homogenous, free flowing powder

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Solubility: Light pink, clear, complete. pH: 7.3. Endotoxin: 1Eu/ml. Directions per Liter: Dissolve 11.75g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Components shown as g/liter: Inorganic salts:. Calcium Chloride 2H2O: 0.165. Cupric Sulfate 5H2O: 0.0000025. Ferrous Sulfate 7H2O: 0.000834. Magnesium Chloride 6H2O: 0.09963. Magnesium Sulfate: 0.02528. Potassium Chloride: 0.305. Potassium Phosphate Mono: 0.06124. Sodium Chloride: 7.517. Sodium Phosphate Dibasic: 0.1324. Zinc Sulfate 7H2O: 0.000863. Amino Acids:. L-Alanine: 0.018. L-Arginine HCl: 0.422. L-Asparagine H2O: 0.03. L-Aspartic Acid: 0.026. L-Cysteine HCL H2O: 0.07026. L-Glutamic Acid: 0.03. L-Glutamine: 0.292. Glycine: 0.016. Powder: L-Histidine HCl H2O: 0.042. L-Isoleucine: 0.0078. L-Leucine: 0.0262. L-Lycine HCl: 0.073. L-Methionine: 0.009. L-Phenylalanine: 0.01. L-Proline: 0.07. L-Serine: 0.021. L-Threonine: 0.0238. L-Tryptophan: 0.004. L-Tyrosine 2Na 2H2O: 0.01586. L-Valine: 0.0234. Vitamins:. L-Ascorbic Acid Na: 0.15. D-Biotin: 0.0000073. Choline Chloride: 0.01396. Vitamin B-12: 0.00136. Folic Acid: 0.00132. myo-Inositol: 0.01802. Niacinamide: 0.00004. D-Pantothenic Acid, Ca: 0.000238. Pyridoxine HCl: 0.00006. Riboflavin: 0.00004. Thiamine HCl: 0.000337. Other: . Thymidine: 0.0007. D-Glucose: 1.802. Hypoxanthine: 0.00404. Linoleic Acid: 0.00009. Thioctic Acid: 0.000206. Phenol Red, Sodium: 0.00125. Putrescine 2HCl: 0.000161. Pyruvic Acid, Sodium: 0.22. Total: 11.75 g/liter

Culture Media; Media-Cell Culture, Nutrient Mixture Ham's

Ham's Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham's F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.

25 L

285 USD

100 L

390