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RPMI 1640 Medium w/o L-Glutamine, Calcium Free (Powder)

MBS652645

10 L

195 USD

Culture Media

RPMI 1640 Medium w/o L-Glutamine, Calcium Free (Powder)

RPMI 1640 Medium w/o L-Glutamine, Calcium Free

Cell Culture Grade

Orange to pink, homogeneous, free flowing powder

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

Endotoxin Level: < or = 1EU/ml

pH: 8.9. Solubility: Magenta, clear, complete. Directions per Liter: I Dissolve 1 0.04g in 900ml of ddH20, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers. Note: It may be necessary to lower the pH to 4.0 with 1 N HCI to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 11 N NaOH prior to the addition of sodium bicarbonate. Inorganic Saits: Magnesium Sulfate: 0.04884. Potassium Chloride: 0.4. Sodium Chloride: 6. Sodium Phosphate Dibasic: 0.8 . Powder: Components shown as g/Liter Amino Acids:. L-ArginineHCI: 0.242. L-Asparagine•H20: 0.0568. L-Aspartic Acid: 0.02. L-Cystine•2HCI: 0.0652. L-Glutamic Acid: 0.02. Glycine: 0.01. L-Histidine•HCI•H20: 0.020. Hydroxy-L -pro line: 0.02. L –Isoleucine: 0.05. L-Leucine: 0.05. L-Lysine•HCI: 0.04. L-Methionine: 0.015. L-Phenylalanine: 0.Ql5. L-Proline: 0.02. L-Serine: 0.03. L-Threonine: 0.02. L-Tryptophan: 0.005. L-Tyrosine•HCI: 0.024. L-Valine: 0.02. Vitamins: . p-Aminobenzoic Acid: 0.001. D-Biotin: 0.0002. Choline Chloride: 0.003. Cyanocobalamin: 0.000005. Folic Acid: 0.001. myo-Inositol: 0.035. Niacinamide: 0.001. D-Pantothenic Acid, Na: 0.00025. Pyridoxine•HCI: 0.001. Riboflavin: 0.0002. Thiamine•HCI: 0.001. Other: . D-Glucose: 2.0. Glutathione: 0.001. Phenol Red, Sodium: 0.005. Total: . 1 0.04g/Liter

Culture Media; Media-Cell Culture, RPMI

RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

10 L

195 USD

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