cDNA

Tissue cDNA, First Strand, Human Adult Normal, Tonsil, BioGenomics

MBS652456

10 Tests

440 USD

cDNA Clone

Tissue cDNA, First Strand, Human Adult Normal, Tonsil, BioGenomics

[Tissue cDNA, First Strand Adult Normal, Tonsil, BioGenomics]

Molecular Biology Grade

Supplied as a liquid in 50mM Tris-Cl, pH 8.3, 75mM potassium chloride, 3mM MgCl2, 10mM DTT.

~2.5ng/ul

Store cDNAs at -20 degree C

Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing

Bioselect Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. Quality Control: 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH7.5). The ratio of 28s/18s is >= 1. 2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR. 3. The synthesized human, animal, and cell line cSDNA was 5’ selected to ensure its full length. The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel. Beta-actin control primer is included. b-Acting Control primer (included). Unit: 1ul cDNA is good for on PCR reaction. Donor Information: DNA from 54 years old, normal, male, human tonsil. Control PCR Condition: Ready First Strand cDNA: 1.0ul. 10 X PCR Buffer: 2.5ul. 10mM dNTP: 0.5ul. Control Primers (5uM): 1.0ul. H2O, Neclease-free: 19.8ul. Tag Polymerase (5u/ul): 0.2ul. PCR Thermocycling: 94 degree C x 2 minutes, 1 cycle. 94 degre C x 30 seconds , 55 degree C x 30 seconds, 72 degree C x 30 seconds, 35 cycles. 72 degree C x 5 minutes, 1 cycle. Then hold at 4 degree C.

Features: Ready to use for PCR. Oligo dT primer used to ensure the entire 3' end of cDNA is present. With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA. The largest selection of cDNAs from different tissues on the market. Documentation of tissues' clinical histories available (additional cost). Uniques Features of cDNA: PCR Ready First Strand cDNA is made from our high-quality total RNA. Each cDNA is a premade, tissue-specific "pool" of first strand cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find not only polymorphic forms of mRNA but also mRNA belonging to a multigene family. The following features have been integrated into our cDNA products to ensure their superior quality.

Cloning; Cloning-cDNA

10 Tests

440 USD