product summary
Loading...
company name :
MyBioSource
product type :
other
product name :
Tissue cDNA, First Strand, Human Adult Normal, Brain, Hippocampus, BioGenomics
catalog :
MBS651929
quantity :
10 rxn
price :
415 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS651929
products type :
cDNA
products full name :
Tissue cDNA, First Strand, Human Adult Normal, Brain, Hippocampus, BioGenomics
products short name :
Tissue cDNA, First Strand Adult Normal, Brain, Hippocampus, BioGenomics
purity :
Molecular Biology Grade
form :
Supplied as a liquid in 1 X RT Buffer: 50mM Tris-CI, I2H 8.3, 75mM KCl, 3mM MgCl2, 10mM DTI.
concentration :
2.5ng/ul
storage stability :
Store cDNAs at -20 degree C
app notes :
Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing
other info1 :
Quality Control: 1 . The Integrity of the RNA used for cDNA syntnesls is examined by' visual inspedion for the I2resence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spedrophotometer. A260/280 is between 1.8 and 2.0 (detected in 1 OmM Tris-CI, pH 7.5). The ratio of 28S/18S is >=1. 2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR. 3. The synthesized eDNA was 5' seleded to ensure its full length. The cDNA was used as template for PCR amplification of b-actin gene and an 838bp b-adin band was visualized on 2% agarose gel. b-Adin control primer is included.
other info2 :
Unit: 1 ul cDNA is sufficient for one PCR readion. Donor Information: Human, male, 82 years old, normal, brain, hippocampus (lot specific). Control PCR Condition: Ready First Strand cDNA: 1 ul. lOX PCR Buffer: 2.5ul. 10mM dNTP: O.5ul. Control Primers (SuM): 1 ul. H20, Nuclease-free: 19.8ul. Taq Polymerase (5u/ul): 0.2ul. PCR Thermocycling:. • 94 degree C x 2 minutes 1 cycle. • 94 degree C x 30 seconds, 55 degree C x 30 seconds, . 72 degree C x 30 seconds, 35 cycles. • 72 degree C x 5 minutes, 1 cycle. Then hold at 4 degree C
products categories :
Cloning; Cloning-cDNA
products description :
Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 1 1 ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul tinal volume. RT Reaction stol2ped by heati.ng at 65 degree C for 10 minutes. The cDNA IS In 1 X RT buffer. (1 X RT Buffer: 50mM Tris-CI, pH 8:1. 75mM KCI, 3mM MgCI2, 10mM D I I). The estimated cDNA concentration is about 2.5ng/ul. 1 ul cDNA is sufficient for one PCR readion.
size1 :
10 rxn
price1 :
415 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!