protein

Heparan Sulfate, Na Salt, Bovine

MBS651827

1 mg

475 USD

Native Protein

Heparan Sulfate, Na Salt, Bovine

[Heparan Sulfate, Na Salt, Bovine]

[Bovine Kidney; Heparitin Sulfate; Heparin Monosulfate; N-Acetylheparan Sulfate.]

Bovine Kidney.

Store at 4°C.

Apperance: White powder. Purity (TLC): Single band. Solubility (Turbidity 1mg/ml in water): Colorless, clear. Total Nitrogen: 3.3%. Total Sulfur: 6%. IR Spectrum: Conforms to structure

Molecular Biology; MB-Carbohydrates, Glycoproteins

Heparan sulfate, bovine, is specially prepared from bovine kidney, purified by a combination of chromatographic and chondroitinase ABC treatment. Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) -D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS-(1->4)- -D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4)alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc(1->4)- -D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are found close to N-sulfates enhancing clustering of sulfate residues and heterogeneity in composition.

1 mg

475 USD

5 mg

1070