protein

Mucins

MBS651782

100 mg

310 USD

Native Protein

Mucins

[Mucins]

Bovine submaxillary gland

Molecular Biology Grade

Supplied as a lyophilized powder

Stable for 6 months at 4ºC. Protect from moisture.

Suitable as a substrate for neuraminidase assays.

Quality Control: SDS-PAGE. Purity Note: Mucin is prepared by the method of Nisizawa and Pigman [The composition and Properties of the Mucin Clot from Cattle Submaxillary Glands, Arch. Oral Biol., 1:16 (1959)]. Bovine sub-maxillary glands are blended in a phosphate buffer, the material is filtered followed by a pH adjustment, then centrifuged and the ppt is resuspended; a final pH adjustment is made and the material is put in dialysis prior to lyophilization.

Molecular Biology; MB-Carbohydrates, Glycoproteins

Mucins are glycoproteins abundant with O-linked oligosaccharides secreted by epithelial mucous membranes. Their protective function is due to their high viscosity. Bovine submaxillary mucin has a molecular weight of 4-40 X 10e5 daltons. The molecule consists of major and minor components with a protein moiety (36.6% of the molecule) and a carbohydrate moiety (56.7% of the molecule). Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands.

100 mg

310 USD

500 mg

515