protein

Chondroitin Sulfate, Proteoglycan Mixture (CSPG)

MBS651750

0.1 mg

500 USD

Carbohydrate/Glycoprotein

Chondroitin Sulfate, Proteoglycan Mixture (CSPG)

Chondroitin Sulfate, Proteoglycan Mixture

Isolated from whole 12-14 day old embryonic chicken brains.

95% by PAGE analysis; Migrates as a large HMW (< 400kD) mass under reduced conditions. Mixture of large, extracellular chondroitin sulfate proteoglycans purified from embryonic chicken brains by chromatrographic methods. Following chondroitinase treatment

Supplied as a liquid in PBS. No preservatives added.

1mg/ml

For long term storage, store at -20 degree C. Aliquots are stable for at least 12 months at - 20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Biology; MB-Carbohydrates, Glycoproteins

100ug protein as determined using the bicinchoninic acid (BCA) assay. Note that 100ug is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans. Contains a mixture of large, extracellular chondroitin sulfate proteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that major components of mixture are neurocan, phosphacan, versican and aggrecan. Aggrecan, versican and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion. Although aggrecan was traditionally thought of as cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four proteoglycans have broad yet distinctive distribution patterns in early embryos. Presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and capable of inhibiting cell-ECM and cell-cell adhesion.

0.1 mg

500 USD