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Ro/SS-A Antigen (Ro-60/SS-A)

MBS651599

100 Units

570 USD

Antigen

Ro/SS-A Antigen (Ro-60/SS-A)

[Ro/SS-A Antigen]

Bovine spleen thymus

Purified by immunoaffinity chromatography using inmobilized anti-SSAA-A(Ro)immunoglobulins. Other antigens (Sm, RNP, Scl-70, Jo-1) are not detected in ELISA assays. Homogeneity is verified by SDS PAGE.

Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide.

0.158 mg/mL

Aliquot and store at -70+/-10°C. Aliquots are stable for 6 months after receipt at 70+/-10°C.Avoid freeze-thaw cycles. Note: Product is unstable above 0°C.For maximum recovery of product, centrifuged the original vial after thawing and prior to removing the cap. Shipping: Ships with Dry Ice.

Suitable for use in ELISA. Other applications not tested.

Optimal dilutions to be determined by the researcher.

SDS-Page

Activity: 1000u/ml

Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for ELISA. Activity will vary with assay conditions/ protocols. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic. Also Available: MBS611481: Ro/SS-A Antigen Pab Hu x Hu. MBS651599: Ro/SS-A Antigen

Molecular Biology; MB-Autoimmune

The (Ro) SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases (1). The antigenic activity of the (Ro) SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA (1). Anti-(Ro) SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the (Ro)SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-(Ro) SS-A have been detected in as high as 96% of Sjogren's Syndrome patients (2), 49% of patients with Systemic Lupus Erythematosus (2), 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-(Ro) SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population (3). In addition, it has been shown that 70% of patients with (Ro) SS-A precipitating antibodies also have rheumatoid factor (4). The titer of rheumatoid factor and the concentration of Anti-(Ro) SSA have been closely correlated in Primary Sjogren's Syndrome (4). Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.

100 Units

570 USD

1000 Units

950