catalog number :
MBS651383
products type :
Antibiotic
products full name :
Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT)
products short name :
[Nourseothricin]
other names :
[nourseothricin acetyltransferase; nourseothricin acetyltransferase]
other gene names :
[RB10252]
form :
Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
app notes :
Suitable for use in selection of recombinant Leishmania strains. Dilution: Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.
image1 heading :
Testing Data
image2 heading :
Testing Data
image3 heading :
Structure
other info1 :
CAS Number: 96736-11-7. Components D&F (HPLC): 89.9%. Components D&E (HPLC): 10.2%. Biological Activity: 110% standard Nr.82. Ash: 2.1% of material. Elementary Analysis: C: 32.3%. H: 5.7%. N: 14.0%. S: 6.7%
other info2 :
Reconstitution: Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.
products categories :
Biochemicals; Biochem-Antibiotics
products description :
A group of antibiotic substances produced by Streptomyces variants. They are composed of an aminosugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of -amino group of the -lysine by Nouresothricin N-acetyltransferase the product of the sat1 gene.
ncbi acc num :
NP_869330.1
ncbi gb acc num :
NC_005027.1
ncbi pathways :
Aminobenzoate Degradation Pathway (13276); Benzoate Degradation Pathway (13243); Benzoate Degradation Pathway (329); Limonene And Pinene Degradation Pathway (13300); Limonene And Pinene Degradation Pathway (411); Microbial Metabolism In Diverse Environments Pathway (179929); Trehalose Biosynthesis, D-glucose 1P = Trehalose Pathway (665672); Trehalose Biosynthesis, D-glucose 1P = Trehalose Pathway (672429); Tyrosine Metabolism Pathway (13239); Tyrosine Metabolism Pathway (325)