TWEAK, Recombinant, Human (TNF-related Weak Inducer of Apoptosis, APO3 Ligand, APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, HCG1991317, MGC129581, MGC20669, Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12, UNQ181/PRO207) | MyBioSource MBS650785 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TWEAK, Recombinant, Human (TNF-related Weak Inducer of Apoptosis, APO3 Ligand, APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, HCG1991317, MGC129581, MGC20669, Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12, UNQ181/PRO207)

catalog :

MBS650785

quantity :

0.005 mg

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS650785

products type :

Recombinant Protein

products full name :

products short name :

TWEAK

other names :

tumor necrosis factor ligand superfamily member 12 proprotein; Tumor necrosis factor ligand superfamily member 12; tumor necrosis factor ligand superfamily member 12; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; tumor necrosis factor (ligand) superfamily, member 12; APO3 ligand; TNF-related weak inducer of apoptosis

other gene names :

TNFSF12; TNFSF12; APO3L; DR3LG; TWEAK; APO3L; DR3LG; TWEAK

uniprot entry name :

TNF12_HUMAN

host :

E Coli

sequence :

MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH

purity :

Molecular Biology Grade. 98% by SDS-PAGE and HPLC.

form :

Supplied as a lyophilized poder from 10mM sodium phosphate, pH 7.5, 0.1 M L-Arg.

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions acan be made in assay buffer.

other info1 :

Biological Activity: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC (peripheral blood mononuclear cells) is less than 10ng/ml. TWEAK wealy induces the death of HT29 cells when cultured in the presence of IFN-y. The ED50 fro this effect if between 30-45 ng/ml. Country of Origin: USA. Endotoxin Level: 0.1ng/ug (1EU/ug)

other info2 :

Reconstitution: We recommend a quick spin followed by reconstitution in 10mM sodium phosphate pH 7.5 to a concentration of 1mg/ml.

products categories :

Growth Factors, Cytokines; Growth Factors-Apoptosis

products description :

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. Recombinant Human TWEAK is a 17.0kD protein, comprising the TNF-like extracellular domain of TWEAK.

ncbi gi num :

4507597

ncbi acc num :

NP_003800.1

ncbi gb acc num :

NM_003809.2

uniprot acc num :

O43508

ncbi mol weight :

27,216 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Gene Expression Pathway (105937); Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway (187211); Stabilization Of MRNA By HuR Pathway (187216); TWEAK Signaling Pathway (672452)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

uniprot summary :

Function: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. Ref.7 Ref.10. Subunit structure: Homotrimer . Probable. Interacts with the angiogenic factor AGGF1/VG5Q. Ref.8 Ref.10. Subcellular location: Cell membrane; Single-pass type II membrane protein Ref.3. Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted Ref.3. Isoform TWE-PRIL: Cell membrane; Single-pass membrane protein Ref.3. Tissue specificity: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing. Sequence similarities: Belongs to the tumor necrosis factor family. Sequence caution: The sequence AAH19047.1 differs from that shown. Reason: Frameshift at position 125.

size1 :

0.005 mg

price1 :

275 USD

size2 :

0.025 mg

price2 :

340

more info or order :

MyBioSource product webpage