protein

Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

MBS650624

0.02 mg

640 USD

Recombinant Protein

Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

[Tumor Necrosis Factor alpha, Rat]

[tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aCleaved into the following 6 chains:Tumor necrosis factor, membrane form; Alternative name(s):; N-terminal fragment]

[Tnf; Tnf; Tnfa; RATTNF; TNF-alpha; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2]

TNFA_RAT

E.coli

Highly Purified >98% (SDS-PAGE and HPLC)

Supplied as a lyophilized powder from PBS. Reconstitute with 200ul sterile ddH2O.

~0.1 mg/ml (after reconstitution)

May be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EIA), Western Blot(WB), Functional Assays

ELISA: 0.2-0.4ng/well. Western Blot: 1.5-3.0ng/lane. Optimal working dilutions to be determined by researcher.

Endotoxin: < 0.1 ng/ug

Growth Factors, Cytokines; Growth Factors-TNF

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Biological Properties: Recombinant rat TNF alpha was tested in a cytoxicity assay on murine L929 cells-this batch demonstrated an ED50 of < 0.5ng/ml.

6981662

NP_036807.1

NM_012675.3

P16599

17.3kD

Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167311)

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells . By similarity. Subunit structure: Homotrimer. Interacts with SPPL2B . By similarity. Subcellular location: Cell membrane; Single-pass type II membrane protein . By similarity. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein . By similarity. Tumor necrosis factor, soluble form: Secreted . By similarity. C-domain 1: Secreted . By similarity. C-domain 2: Secreted . By similarity. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space . By similarity.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1 . By similarity.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid . By similarity. Sequence similarities: Belongs to the tumor necrosis factor family.

0.02 mg

640 USD