protein

SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)

MBS650316

0.002 mg

310 USD

Recombinant Protein

SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)

SCF

stem cell factor

The sequence of the first five amino acids was determined to be Met-Glu-Gly-Ile-Cys. Biological Activity:. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <2ng/ml, corresponding to a Specific Activity of 5x10e5IU/mg.

Purified. Purified by chromatography.

Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute with 20ul sterile ddH2O. For long term storage, add a carrier protein (0.1% HSA or BSA).

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Growth Factors, Cytokines; Growth Factors-Cell Factors

Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.

1246100

AAB35922.1

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q22. Cellular Component: extracellular space; cytoskeleton; cytoplasm; plasma membrane; extracellular region; integral to membrane. Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; signal transduction; cell proliferation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; innate immune response; positive regulation of Ras protein signal transduction; positive regulation of myeloid leukocyte differentiation; cell adhesion; neural crest cell migration; positive regulation of DNA replication. Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7

0.002 mg

310 USD

0.01 mg

440