CES2 (Cocaine Esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate Deacetylase 2, ICE) | MyBioSource MBS647447 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CES2 (Cocaine Esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate Deacetylase 2, ICE)

catalog :

MBS647447

quantity :

0.1 mg

price :

655 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[12K61]

reactivity :

human

more info or order :

MyBioSource product webpage

image

image 1 :

Detection of Human CES2 by Western Blot. Western Blot shows lysates of human liver tissue. PVDF membrane was probed with 2ug/ml of MBS647447 followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for CES2 at ~62kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

image 2 :

CES2 in HepG2 Human Cell Line. CES2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using MBS647447 (10ug/ml) for 3 hours at RT. Cells were stained using the NorthernLightsTM 557-conjugated Mouse IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.

product information

catalog number :

MBS647447

products type :

Antibody

products full name :

CES2 (Cocaine Esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate Deacetylase 2, ICE)

products short name :

[CES2]

products name syn :

[Anti -CES2 (Cocaine Esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate Deacetylase 2, ICE)]

other names :

[cocaine esterase isoform 2; Cocaine esterase; cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2; carboxylesterase 2; Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56)]

other gene names :

[CES2; CES2; iCE; CE-2; PCE-2; CES2A1; ICE; CE-2; hCE-2]

uniprot entry name :

EST2_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[12K61]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human CES2. Does not react with recombinant mouse Carboxylesterase 2.

purity :

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200 uL sterile PBS.

concentration :

~0.5 mg/ml (after reconstitution)

storage stability :

Lyophilized and reconstituted products are stable dor 12 months after receipt at -20°C. Reconsitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Suitable for use in Western Blot (WB), Immunoprecipitation (IP) and Immunocytochemistry (ICC). Other applications not tested.

app notes :

Western Blot (WB): 2 ug/ml . Immunocytochemistry: 8-25 ug/mL using immersion fixed . HepG2 human hepatocellular carcinoma cell line. Immunoprecipitation: 25 ug/mL using conditioned cell culture spiked with recombinant human CES2. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Testing Data

other info1 :

Grade: Affinity purified. Immunogen: Recombinant protein corresponding to Gln27-leu 559 (predicted) of human CES2 expressed in NSO cell line.

products categories :

Antibodies; Abs to Enzymes

products description :

Carboxylesterase 2 is a member of a serine esterase family composed of enzymes which hydrolyze ester and amide bonds. The members in this family share the serine hydrolase fold observed in other esterases. They have broad substrate specificity from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. They play a major role in the pharmacokinetics of most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in the detoxification of drugs such as cocaine and heroin in serum and liver. In addition to narcotics, they can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification. This reaction is important for cholesterol homeostasis. Three major human CESs have been identified. CES1 is highly expressed in liver. CES2 is present in the small intestine, colon, kidney, liver, heart, brain, and testis. CES3 is brain-specific. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia.

ncbi gi num :

37622887

ncbi acc num :

NP_932327.1

ncbi gb acc num :

NM_198061.2

uniprot acc num :

O00748

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Irinotecan Pathway (198905); Phase I, Non P450 Pathway (198854); Retinol Biosynthesis Pathway (545293)

ncbi summary :

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]

uniprot summary :

Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Ref.2. Catalytic activity: Cocaine + H2O = ecgonine methyl ester + benzoate. Ref.2A carboxylic ester + H2O = an alcohol + a carboxylate. Ref.24-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate. Ref.2. Subunit structure: Monomer. Ref.2. Subcellular location: Endoplasmic reticulum lumen . By similarity. Tissue specificity: Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum. Ref.1. Post-translational modification: Glycosylated. Ref.2. Sequence similarities: Belongs to the type-B carboxylesterase/lipase family. Biophysicochemical propertiesKinetic parameters:KM=0.39 mM for cocaine Ref.2KM=0.15 mM for 4-methylumbelliferyl acetateKM=6.8 mM for heroinKM=0.13 mM for 6-monoacetylmorphine. Sequence caution: The sequence AAH32095.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAW29943.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD28531.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD98009.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

size1 :

0.1 mg

price1 :

655 USD

more info or order :

MyBioSource product webpage