antibody

Goat Anti-NALP3, ID

MBS645146

0.1 mg

570 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, ELISA, immunohistochemistry

Antibody

Goat Anti-NALP3, ID

[NALP3]

[Goat Anti-NALP3, ID (NACHT-, LRR- and PYD-Containing Protein 3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein, PYPAF1, PYRIN-Containing APAF1-like Protein 1, CIAS1, Caterpiller Protein 1.1, CLR1.1, C1orf7, CIAS1, NLRP3, PYPAF1, Angiotensin/Vasopressin Receptor AII/AVP-like]

[NACHT, LRR and PYD domains-containing protein 3 isoform e; NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; NLR family, pyrin domain containing 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1]

[NLRP3; NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1]

NALP3_HUMAN

Polyclonal

IgG

Goat

Bovine, Canine, Human, Mouse, Porcine, Rat

Recognizes human NLRP3. Expected to recognize all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1).

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

0.5 mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA, Western Blot, Immunohistochemistry

Peptide ELISA: 1:64,000 (detection limit) Western Blot: 0.3ug/ml detects a band at ~70kD in humn lymph node and tonsil lysates. Immunohistochemistry: 2-4ug/ml Requires HIER using sodium citrate buffer pH6.0. Shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts in paraffin embedded human tonsil. Optimal dilutions to be determined by the researcher.

Immunohistochemistry (IHC)

Immunogen: Peptide corresponding to C-DLYEKAKRDEPK, from an internal region of human NLRP3. Species sequence homology: bovine, canine, mouse, porcine, rat

Antibodies; Abs to Inflammasomes

NALP3 is a cytoplasmic, pyrin-like protein belonging to the NLRP family with a DPIN domain, seven leucine rich repeats and a NACHT domain. It is known to interact with apoptosis-associated speck-like protein PYCARD/ASC and is a part of the NALP3 inflammasome complex. NALP3 is involved in the activation of caspase-1 and caspase-5 in response to a number of triggers including bacterial or viral infection, thus leading to the processing of IL-1B and IL-18. It may also inhibit TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65 and function in the activation of NF-kappa-B signaling, thus regulating inflammation and apoptosis. Expression is predominantly in peripheral blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes. NALP3 defects leads to several diseases including familial cold auto inflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).

341865590

NP_004886.3

NM_001243133.1

Q96P20

118,173 Da

Immune System Pathway (106386); Inflammasomes Pathway (366166); Influenza A Pathway (217173); Influenza A Pathway (217150); Innate Immune System Pathway (106387); NOD Pathway (198792); NOD-like Receptor Signaling Pathway (122191); NOD-like Receptor Signaling Pathway (122131); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (366164); Pertussis Pathway (218111)

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 1q44. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; peptidoglycan binding; ATP binding. Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; negative regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; innate immune response; interleukin-18 production; interleukin-1 secretion; inflammatory response; detection of biotic stimulus; defense response to virus. Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome

0.1 mg

570 USD