catalog number :
MBS644440
products full name :
Vaccinia Virus, A27L
products short name :
Vaccinia Virus, A27L
products name syn :
Anti -Vaccinia Virus, A27L
specificity :
Recognizes free virus and infected cells. Specific to the A27L protein. Positive for the Lister and NYCBH strains of vaccinia.
purity :
Affinity Purified. Purified by Protein A affinity chromatography.
form :
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
concentration :
0.1 mg/ml
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
Suitable for use in ELISA, and Immunofluorescence. Other applications not tested. Recommended Dilution:. Immunofluorescence: 1:10-1:50. ELISA: 1:20-1:200. Optimal dilutions to be determined by the reseacher.
other info1 :
Immunogen: Partially purified Vaccinia virus.
products categories :
Antibodies; Abs to Infectious Disease
products description :
Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KD protein (A27L). The N terminus of this protein recognizes heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KD protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.