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Tissue, Total RNA, Human Adult Normal, Lymph node, BioGenomics

MBS638752

0.01 mg

400 USD

RNA Extraction

Tissue, Total RNA, Human Adult Normal, Lymph node, BioGenomics

[Tissue, Total RNA Adult Normal, Lymph node, BioGenomics]

Total RNA isolated by modified guanidine thiocyanate techniques

Supplied as a liquid in RNA storage buffer

1.27 mg/mL

Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Shipping: Ships with dry ice.

Suitable for use in Northern Blot, RT-PCR, RACE, cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection and primer extension, RNA display, purification of mRNA.

Human

Features: Total RNA isolated from a wide variety of hard to obtain tissues. Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA. Extensive quality control procedures to ensure high quality. High efficiency reverse transcription. Available documentation of tissues' clinical histories (additional cost). Donor Information: RNA from one, normal, adult, 29 year-old male, human lymph node. Quality Control: 1. The integrity of the RNA is examined by visual insepction for the presence of intact bands of 18s and 28s RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotomer. (A260/280= 1.8-2.0 detected in 10mM Tris-Cl, pH 7.5). 2. The RNA is treated by DNAse I. lis is tested as DNA free RNA by PCR. 3. cDNA synthesis is successfully performed by using this RNA as a template.

Cells, Lysates, Plasma, Serum, Tissues; Tissues-Total RNA

Total RNAs are isolated from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, and RNA differential display. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1/1 between 28S and 18S ribosomal RNA. We guarantee high efficiency reverse transcription.

0.01 mg

400 USD