Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11) | MyBioSource MBS638161 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)

catalog :

MBS638161

quantity :

0.05 mg

price :

740 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS638161

products type :

Recombinant Protein

products full name :

Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)

products short name :

[Collectin 11]

other names :

[collectin-11 isoform a; Collectin-11; collectin-11; collectin kidney protein 1; collectin sub-family member 11; Collectin kidney protein 1]

other gene names :

[COLEC11; COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; CL-K1]

uniprot entry name :

COL11_HUMAN

purity :

>85% as determined by SDS-PAGE under reducing conditions and visualized by silver strain.

form :

Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml.

storage stability :

Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Western Blot (WB). Other applications not tested

app notes :

Dilution: Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined by the researcher.

other info1 :

Activity: Measured by its ability to bind Alpha-L-Fucose. Endotoxin Level: <1EU per 1ug of protein (LAL)

products categories :

Molecular Biology; MB-Binding Proteins

products description :

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1-3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D. DNA sequence encoding mouse CL-K1 (Gln 27-Leu 272; Accession # AAI0438) was fused to a 6x polyhistidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.

ncbi gi num :

13128972

ncbi acc num :

NP_076932.1

ncbi gb acc num :

NM_024027.4

uniprot acc num :

Q9BWP8

ncbi mol weight :

33-38kD

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Phagosome Pathway (153910); Phagosome Pathway (153859); Scavenging By Class A Receptors Pathway (833814)

ncbi summary :

This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

COLEC11: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration. Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2). 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the COLEC10/COLEC11 family. 9 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p25.3. Cellular Component: collagen; extracellular region. Molecular Function: mannose binding. Biological Process: receptor-mediated endocytosis; multicellular organismal development; developmental process. Disease: 3mc Syndrome 2

size1 :

0.05 mg

price1 :

740 USD

more info or order :

MyBioSource product webpage