IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO) | MyBioSource MBS637936 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO)

catalog :

MBS637936

quantity :

0.05 mg

price :

650 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS637936

products type :

Recombinant Protein

products full name :

IDO, Recombinant, Mouse (His) (Indoleamine 2,3-dioxygenase, INDO)

products short name :

[IDO]

other names :

[indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase]

other gene names :

[IDO1; IDO1; IDO; INDO; IDO-1; IDO; INDO; IDO-1]

uniprot entry name :

I23O1_HUMAN

host :

E.Coli

sequence :

Mouse IDO (aa 1-407) is fused at the C-terminus to a His-tag.

purity :

Highly Purified. >=90% (SDS-PAGE)

form :

0.2um-filtered solution in 50mM TRIS-Cl, pH 7.4, 1mM EDTA.

concentration :

1mg/ml

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

other info1 :

Biological Activity: Specific Activity: >100,000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.

other info2 :

Endotoxin: <1EU/ug (LAL). Heme Content: Not determined for end product. 7uM hermin was adde for protein induction.

products categories :

Molecular Biology; MB-Enzymes, Oxidase

products description :

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Recombinant protein corresponding to mouse IDO (aa1-407), at C-terminus fused to His-tag.

ncbi gi num :

4504577

ncbi acc num :

NP_002155.1

ncbi gb acc num :

NM_002164.5

uniprot acc num :

P14902

ncbi mol weight :

~40kD (SDS-PAGE)

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Tryptophan Catabolism Pathway (106190); Tryptophan Metabolism Pathway (82964); Tryptophan Metabolism Pathway (198850); Tryptophan Metabolism Pathway (332); Tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway (139210)

ncbi summary :

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

uniprot summary :

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family. Protein type: Amino Acid Metabolism - tryptophan; Cell cycle regulation; Oxidoreductase; EC 1.13.11.52. Chromosomal Location of Human Ortholog: 8p12-p11. Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytosol. Molecular Function: electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity. Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction

size1 :

0.05 mg

price1 :

650 USD

more info or order :

MyBioSource product webpage