Myosin IIA, non-muscle, heavy chain (Recombinant) (Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A) | MyBioSource MBS637834 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Myosin IIA, non-muscle, heavy chain (Recombinant) (Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A)

catalog :

MBS637834

quantity :

0.1 mg

price :

545 USD

more info or order :

MyBioSource product webpage

image

image 1 :

Schematic antibody structure.

image 2 :

Immunofluorescence/Immunocytochemistry analysis of human Myosin IIA (non-muscle) (heavy chain) using MBS637834. Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with MBS637834 (1ug /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.

product information

catalog number :

MBS637834

products type :

Recombinant Protein

products full name :

Myosin IIA, non-muscle, heavy chain (Recombinant) (Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A)

products short name :

[Myosin IIA, non-muscle, heavy chain]

products name syn :

[Anti -Myosin IIA, non-muscle, heavy chain (Recombinant) (Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A)]

other names :

[myosin-9; Myosin-9; myosin-9; myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; myosin heavy chain, non-muscle IIa; cellular myosin heavy chain, type A; non-muscle myosin heavy polypeptide 9; myosin, heavy chain 9, non-muscle; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa]

other gene names :

[MYH9; MYH9; MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA; NMMHC-A; NMMHC II-a; NMMHC-IIA]

uniprot entry name :

MYH9_HUMAN

clonality :

Monoclonal

isotype :

IgG2,l

clone :

[SF9]

host :

Human

reactivity :

Mouse, human, drosophila.

specificity :

Recognizes rat myosin IAA (heavy chain).

purity :

Highly Purified. Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).

form :

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

concentration :

1 mg/ml

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in Electron Microscopy, ELISA, Immunocytochemistry and Western Blot. Other applications not tested.

app notes :

Immunocytochemistry: 1:1000. Western Blot: 1:1000. Optimal dilutions to be determined by researcher.

image1 heading :

Testing Data

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Full length myosin IIA from rat liver.

products categories :

Antibodies; Abs to Myosin

products description :

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

ncbi gi num :

12667788

ncbi acc num :

NP_002464.1

ncbi gb acc num :

NM_002473.4

uniprot acc num :

P35579

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (771577); Immune System Pathway (106386); Innate Immune System Pathway (106387); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway (771579); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

ncbi summary :

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

uniprot summary :

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: cortical cytoskeleton; signalosome; protein complex; myosin II complex; leading edge; contractile ring; actomyosin; immunological synapse; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; stress fiber; plasma membrane; spindle; uropod; neuromuscular junction; integrin complex; nucleus; cleavage furrow. Molecular Function: actin filament binding; microfilament motor activity; calmodulin binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; ADP binding; actin-dependent ATPase activity; actin binding; ATP binding. Biological Process: integrin-mediated signaling pathway; axon guidance; monocyte differentiation; blood vessel endothelial cell migration; in utero embryonic development; actin filament-based movement; actomyosin structure organization and biogenesis; membrane protein ectodomain proteolysis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; platelet formation; angiogenesis; leukocyte migration; meiotic spindle organization and biogenesis; myoblast fusion. Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

size1 :

0.1 mg

price1 :

545 USD

more info or order :

MyBioSource product webpage