protein

Matrix Metalloproteinase 3, Recombinant, Human (MMP-3, Stromelysin-1, SL-1, Transin)

MBS637729

0.002 mg

275 USD

Recombinant Protein

Matrix Metalloproteinase 3, Recombinant, Human (MMP-3, Stromelysin-1, SL-1, Transin)

Matrix Metalloproteinase 3

matrix metalloproteinase 3; Stromelysin-1; stromelysin-1; transin-1; proteoglycanase; matrix metalloproteinase-3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metallopeptidase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MMP-3; Transin-1

MMP3; MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1; STMY1; SL-1; MMP-3

MMP3_HUMAN

HEK293

>95% (SDS-PAGE)

Supplied as a liquid in in Tris, CaCl2, sodium chloride and Brij35.

0.3mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquot are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Biological Activity: The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/ug.

Actication Protocol: 1.Dilute MMP3 to 20ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM sodium chloride, 0.05% and Brij35, pH 7.5. 2. Activate MMP3 by adding Chymotrypsin to final concentration of 5ug/ml. 3. Incubate at 37 degree C for 30 minutes. 4.Stop activation with 2mM PMSF. Pre-warm the PMSF to 37 degree C prior to adding to sample.

Molecular Biology; MB-Matrix Metalloproteinases

Matrix metalloproteinases are important regulators of extracellular matrix molecules and cell-cell signaling and are involved in the regulation of the pericellular space, including the cleavage of matrix proteins. In particular, MMP3 has been shown to be effective in cleaving heparan sulfate proteoglycans. Purified recombinant MMP3 can directly cleave agrin, and it can also remove agrin from synaptic basal lamina. MMP3 activity is itself regulated as activation of MMP3 is lost in denervated muscles. Recombinant Human MMP-3 produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a his-tag at the C-terminus. Matrix Metalloproteinase-3 is purified by proprietary chromatographic techniques.

90287774

ABD92797.1

P08254

52kD

Activation Of Matrix Metalloproteinases Pathway (576264); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (827690); Degradation Of The Extracellular Matrix Pathway (576263); Degradation Of The Extracellular Matrix Pathway (827688); EGFR Transactivation By Gastrin Pathway (645296); Extracellular Matrix Organization Pathway (576262); Extracellular Matrix Organization Pathway (827676)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Protease; EC 3.4.24.17; Secreted. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; mitochondrion; extracellular region; cytosol. Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding. Biological Process: collagen catabolic process; positive regulation of protein oligomerization; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration. Disease: Coronary Heart Disease, Susceptibility To, 6

0.002 mg

275 USD

0.01 mg

340