protein

Hepatitis A Virus, P3C, Recombinant (HAV)

MBS637723

0.1 mg

360 USD

Recombinant Protein

Hepatitis A Virus, P3C, Recombinant (HAV)

Hepatitis A Virus, P3C

E Coli

Immunoreactive with sera of HAV-infected individuals

>90% by 10 PAGE with coomassie staining. Chromatographically purifies.

Supplied as a liquid in 10mM CBB, pH 9.6, 0.1% SDS, 50% glycerol.

1mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant HAV Antigen may be used in ELISA and Western Blot, excellent for detection of HAV with minimal specificity problems. Note: Recombinant protein corresponding to aa1643-1743 containing the P3C immunodominant regions from the Hepatitis A Virus expressed in E.coli.

Molecular Biology; MB-Infectious Disease

Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein and a panel of serum samples from acutely HAV-infected patients. The term "antigenic domain" is used in this study to define a protein region spanned with consecutive overlapping immunoreactive peptides. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. It is interesting to note that four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins.

40kD

0.1 mg

360 USD

0.5 mg

770