protein

5'-Nucleotidase, Recombinant, Mouse (CD73, Ecto-5'nucleotidase, 5-NT)

MBS637721

0.01 mg

650 USD

Recombinant Protein

5'-Nucleotidase, Recombinant, Mouse (CD73, Ecto-5'nucleotidase, 5-NT)

5'-Nucleotidase

5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase

Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT

5NTD_RAT

>90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.

Supplied as a liquid in 20mM Tris, 0.12M sodium chloride, 4mM CaCl2, 20% glycerol, pH 7.5.

0.44mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen: CHO-derived recombinant mouse 5'-Nucleotidase/CD73. Specific Activity: >10,000 pmoles/min/ug. Biological Activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. Endotoxin: <1Eu/mg

Molecular Biology; MB-Enzymes, Nucleotidase

CD73 (Ecto-5'nucleotidase, 5 '-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells. Recombinant mouse 5'-Nucleotidase/CD73 ectodomain (rmCD73) encoded by the NT5E gene was expressed with a C-terminal 6X His tag in a Chinese Hamster Ovary cell line. CD73, an ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5'-nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3). The purified, secreted rmCD73 has a N-terminal sequence of W29ELTILHTND. The 527 amino acid rmCD73 predicts a molecular mass of 59kD and migrates as a 70kD band in SDS-PAGE under reducing conditions.

148747417

NP_067587.2

NM_021576.2

P21588

70kD

Metabolism Pathway (828668); Metabolism Of Nucleotides Pathway (828815); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (828819); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (828816); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (828823); Pyrimidine Metabolism Pathway (83339)

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); EC 3.1.3.5; Membrane protein, integral; Membrane protein, GPI anchor. Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane. Molecular Function: 5 -nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding. Biological Process: dephosphorylation; adenosine biosynthetic process; negative regulation of inflammatory response; brain development; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion

0.01 mg

650 USD