protein

GST-mu, Recombinant, Human (Glutathione S Transferase mu, GSTmu)

MBS637319

0.025 mg

510 USD

Recombinant Protein

GST-mu, Recombinant, Human (Glutathione S Transferase mu, GSTmu)

GST-mu

GST-mu

Human GST-my/GST M1-1, purified, expressed in E.coli.

Highly Purified (>95%)

Supplied as a liquid in 50mM Tris, pH7.5, 50mM sodium chloride; 1mM DTT, 1mM EDTA, 50% glycerol.

1mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in biological activity determination, ELISA, and for standards or for positive control for Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher.

Specific Activity: ~50units/mg. Under native conditions, GST-mu is homodimeric protein of ~50kd and under reducing conditions ~25kD.

Unit Definition: One unit will catalyze the conjugation of 1 micromole of 1-chloro-2-4-dinitrobenzene and reduced glutathione per min at pH 6.5 at 25 degree C.

BioSeparation; BioSep-Assay Detection, Tags

GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y. The GST M1 along with GST M2-GST M5 are mapped to Chrm 1, There is a close physical proximity between GSTM1 and GSTM2 loci, which shares 99% nucleotide sequence identity over 460 nucleotides of 3-prime untranslated mRNA. The 181aa long GST M1 is mainly expressed in liver, brain, testis, kidney and lung and show enzyme activity towards CDNB; AFB1 - epoxide; trans-4-phenyl-3buten-2-one.

337730406

AEI70674.1

~50kD, ~25kD

0.025 mg

510 USD