protein

SFRP3, Recombinant, Mouse (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

MBS637291

0.01 mg

710 USD

Recombinant Protein

SFRP3, Recombinant, Mouse (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

[SFRP3]

[secreted frizzled-related protein 3; Secreted frizzled-related protein 3; secreted frizzled-related protein 3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1; frizzled-related protein; Frezzled; Fritz; Frizzled-related protein 1; FrzB-1]

[FRZB; FRZB; FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN; FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3]

SFRP3_HUMAN

Highly Purified. =>95%, as determined by SDS-PAGE and visualized by silver stain.

Supplied as a lyophilized powder in PBS, pH 7.4, BSA. Reconstitute with 100 uL sterile PBS.

~0.1 mg/mL(after reconstitution)

Lyophilized powder may be stored at -20°C.Stable for 6 months after receipt at -20°C.Reconstitute with sterile PBS.Aliquot to avoid repeated freezing and thawing. Store at -70°C.For maximum recovery of procut, centrifuge the original vial after thawing and prior to removing the cap. Shipping: Ships with dry ice

. Source: A DNA sequence encoding the mature mouse sFRP-3 protein sequence Ala33-Ser323 was fused to a 6X histidine tag at the carboxy-terminus. The fusion protein was expressed in a mouse myeloma cell line, NS0. Swiss/UniProt Accession: P97401. Molecular Mass: Based on N-terminal sequencing, the mature mouse sFRP-3 protein starts at Ala33 and has a calculated molecular mass of 34kD. As a result of glycosylation, the recombinant protein migrates as an ~37kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. ED 50 1.5-6.0 ug/mL. Endotoxin : <1.0 EU per 1 ug (LAL)

Molecular Biology; MB-Wnt Proteins

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. sFRP-3 is often referred to as FRZB, other names also include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The mouse protein shares 76% identity with Xenopus, and 92% with human proteins. The gene for mouse sFRP-3 has been localized to the central region of chromosome 2. Murine sFRP-3 is expressed in the primitive streak during gastrulation, as well as in the retina, foregut diverticulum, nervous system, and posterior mesoderm during development. In adult tissues, sFRP-3 expression, as determined by Northern blot, is detected in the heart, brain, spleen, skeletal muscle, kidney and testis. The N-terminal portion of sFRP-3 protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. sFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA (Hoang et al., 1996, J. Biol. Chem. 271:26131; Leyns et al., 1997, Cell 88:747;

38455388

NP_001454.2

NM_001463.3

Q92765

Adipogenesis Pathway (198832); Endochondral Ossification Pathway (198812)

The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]

FRZB: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. Defects in FRZB are associated with susceptibility to osteoarthritis type 1(OS1). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q32.1. Cellular Component: extracellular space; membrane; integral to membrane. Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity. Biological Process: negative regulation of cell proliferation; convergent extension involved in organogenesis; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of fat cell differentiation; neural crest cell differentiation; negative regulation of cell growth; skeletal development. Disease: Osteoarthritis Susceptibility 1

0.01 mg

710 USD