protein

Carbonic Anhydrase 1, Recombinant Human, aa1-261, His tag (CA1)

MBS637086

0.1 mg

425 USD

Recombinant Protein

Carbonic Anhydrase 1, Recombinant Human, aa1-261, His tag (CA1)

Carbonic Anhydrase 1 Human, aa1-261, His tag

carbonic anhydrase 1; Carbonic anhydrase 1; carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; carbonic anhydrase I; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I

CA1; CA1; CAB; CA-I; Car1; CAB; CA-I

CAH1_HUMAN

Human

MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F

Affinity Purified. >95% (SDS-PAGE) Purified by immunoaffinity chromatography.

Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.

1mg/ml

Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

Molecular Biology; MB-Enzymes

Carbonic anhydrase 1(CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. Recombinant protein corresponding to human Carbonic anhydrase1, his-tagged, expressed in E. coli (NP_001122301).

192447432

NP_001122302.1

NM_001128830.2

P00915

31.0 kDa

C-MYB Transcription Factor Network Pathway (138073); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); O2/CO2 Exchange In Erythrocytes Pathway (645346); Reversible Hydration Of Carbon Dioxide Pathway (645352); Uptake Of Carbon Dioxide And Release Of Oxygen By Erythrocytes Pathway (645347); Uptake Of Oxygen And Release Of Carbon Dioxide By Erythrocytes Pathway (645348)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009]

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase. Chromosomal Location of Human Ortholog: 8q21.2. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process

0.1 mg

425 USD