protein

CD200R1, Fc Chimera, Recombinant, Mouse (OX2R, OX2 Receptor)

MBS637024

0.05 mg

710 USD

Recombinant Protein

CD200R1, Fc Chimera, Recombinant, Mouse (OX2R, OX2 Receptor)

[CD200R1, Fc Chimera]

Recombinant Mouse

85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.

Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

~0.1 mg/mL (after reconstitution)

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Activity: Measured by its ability to bind in a functional ELISA. Endotoxin Level: <0.01 EU/ug

Molecular Mass: Calculated molecular mass of 49.7kD. As a result of glycosylation, the recombinant monomer migrates as an ~ 90-100kD protein in SDS-PAGE under reducing conditions.

Molecular Biology; MB-CD Markers

CD200 R1, also known as OX-2 receptor, is a 90kD, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity.1-3 The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain.4 Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, 5-7 while its ligand, CD200, is widely distributed.8 Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders.9 Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains.10 The CD200 R-like molecules may interact differently with CD200.11, 12 The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals.13-15 CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.4, 16. A DNA sequence encoding the extracellular domain of mouse CD200 R1 (Met 1-Pro 236; Accession # BAC40777) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0

0.05 mg

710 USD