protein

CD163, Recombinant, Human (Hemoglobin/Haptoglobin Complex Receptor, M130 antigen, Ber-Mac3, Ki-M8, SM4)

MBS636721

0.05 mg

650 USD

Recombinant Protein

CD163, Recombinant, Human (Hemoglobin/Haptoglobin Complex Receptor, M130 antigen, Ber-Mac3, Ki-M8, SM4)

CD163

CD163; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen; CD163 molecule; Hemoglobin scavenger receptor

CD163; CD163; M130; MM130; M130; sCD163

C163A_HUMAN

Human

Highly Purified. 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.

Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.

~0.25mg/ml (after reconstitution)

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

Suitable for use Western Blot. Dilution: Western Blot: 110kD migrates as an ~ 160kD protein in SDS-PAGE under reducing conditions.

Structure: Monormer. Endotoxin < or = 1EU: <0.1EU (LAL). Activity: Measured by its ability to bing Haptoglobin/Hemoglobin (Hp/Hb) complexes. Kristiansen, M, et al., Nature, 2001 409:198.

Molecular Biology; MB-CD Markers

CD163, previously called M130 or p155, is a 130-160kD type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39-84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is most abundant, being generally expressed on the cell surface and most active in endocytosis (5). A ~130kD soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7-10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL-10, IL-6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb-Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13-15).

5107945

CAB45233.1

Q86VB7

110kD, migrates at 135-140kD.

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: integral to plasma membrane; plasma membrane; extracellular region. Molecular Function: protein binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; acute-phase response

0.05 mg

650 USD