protein

Ghrelin, Recombinant Human, His-tag, aa24-117 (Obestatin)

MBS636705

0.1 mg

475 USD

Recombinant Protein

Ghrelin, Recombinant Human, His-tag, aa24-117 (Obestatin)

[Ghrelin Human, His-tag, aa24-117]

[ghrelin]

E coli

Human

MGSSHHHHHH SSGLVPRGSH MGSSFLSPEH QRVQQRKESK KPPAKLQPRA LAGWLRPEDG GQAEGAEDEM EVRFNAPFDV GIKLSGVQYQ QHSQALGKFL QDILWEEAKE APADK

Affinity Purified. >90% (SDS-PAGE).

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 10% glycerol.

1mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Endotoxin: < 1EU/ml

Molecular Biology; MB-Hormones, Steroids

Ghrelin, also known as Obestatin, is the ligand for growth hormone secretagogue receptor type 1 (GHSR). It induces the release of growth hormone from the pituitary and involved in growth regulation. This protein has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Ghrelin plays a significant role in neurotrophy, particularly in the hippocampus, and is essential for cognitive adaptation to changing environments and the process of learning. Recombinant Ghrelin protein was expressed in E. coli and purified by using conventional chromatography techniques.

304435649

ADM33790.1

12.8kD

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

0.1 mg

475 USD