protein

RAP, Recombinant, Rat (Receptor Associated Protein)

MBS636670

0.1 mg

695 USD

Recombinant Protein

RAP, Recombinant, Rat (Receptor Associated Protein)

RAP, Rat

rap guanine nucleotide exchange factor 3; Rap guanine nucleotide exchange factor 3; rap guanine nucleotide exchange factor 3; epac 1; cAMP-GEFI; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI); Rap guanine nucleotide exchange factor (GEF) 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; cAMP-regulated guanine nucleotide exchange factor I

Rapgef3; Rapgef3; Epac; Epac; Epac1; EPAC 1; cAMP-GEFI

RPGF3_RAT

E Coli

Purified. >95% pure (SDS-PAGE)

Supplied as a lyophilized powder from TBS, pH 7.5, 0.1% BSA, 0.09% sodium azide. Reconstitute with 100ul ddH2O.

~1mg/ml (after reconstitution)

Lyophilized powder may be stored at -20 degree C for short term only. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -70 degree C. Reconstituted product is stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, immunoblotting and receptor-binding studies. Other applications not tested. Optimal dilutions to be determined by the researcher.

Rat his:RAP: c-myc fusion partner.

Molecular Biology; MB-Receptors

RAP binds with high affinity to certain members of the low-density lipoprotein (LDL) receptor family such as LDL receptor-related protein (LRP), gp330/megalin, and the very low-density lipoprotein (VLDL) receptor and, once bound to these receptors, antagonizes their ligand binding ability. Despite the presence of a putative signal sequence on RAP, this molecule is not secreted but remains cell-associated. RAP is contained primarily within the endoplasmic reticulum and Golgi compartments, with only a trace found at the cell surface and within the endosomal compartments. Genetic deletion of RAP in mice has no effect on LRP mRNA levels but does lead to incomplete processing of LRP in the brain and liver resulting in decreased LRP antigen levels in these organs.

11067419

NP_067722.1

NM_021690.1

Q9Z1C8

40kD

Adaptive Immune System Pathway (829043); Hemostasis Pathway (828446); Immune System Pathway (829042); Integration Of Energy Metabolism Pathway (828783); Integrin AlphaIIb Beta3 Signaling Pathway (828273); Integrin Cell Surface Interactions Pathway (828272); Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Long-term Potentiation Pathway (83477); Long-term Potentiation Pathway (496)

binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A)in a PKA-independent manner [RGD, Feb 2006]

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing. Protein type: GEFs; GEFs, misc. Cellular Component: membrane; cytoplasm; cAMP-dependent protein kinase complex. Molecular Function: Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding. Biological Process: positive regulation of syncytium formation by plasma membrane fusion; positive regulation of protein export from nucleus; positive regulation of angiogenesis; positive regulation of stress fiber formation; angiogenesis; regulation of protein kinase activity; positive regulation of peptidyl-serine phosphorylation; Rap protein signal transduction; positive regulation of GTPase activity

0.1 mg

695 USD