product summary
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company name :
MyBioSource
product type :
antibody
product name :
Tubulin, alpha
catalog :
MBS636455
quantity :
0.1 mg
price :
545 USD
clonality :
monoclonal
host :
human
conjugate :
nonconjugated
clone name :
[F2C]
application :
western blot, immunocytochemistry
more info or order :
image
image 1 :
Human alpha-Tubulin is detected by immunocytochemistry using MBS636455. Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with MBS636455 (1ug /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
image 2 :
Structure of clone F2C recombinant antibody. The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
product information
catalog number :
MBS636455
products type :
Antibody
products full name :
Tubulin, alpha
products short name :
[Tubulin, alpha]
products name syn :
[Anti -Tubulin, alpha]
other names :
[tubulin; Tubulin alpha chain]
other gene names :
[TUBA]
uniprot entry name :
TBA_EUGGR
clonality :
Monoclonal
isotype :
IgG2
clone :
[F2C]
host :
Human
specificity :
Recognizes bovine alpha-tubulin. Spececies Crossreactivity: Human and mouse
purity :
Highly Purified. >95% (SDS-PAGE).
form :
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
concentration :
1mg/ml
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
tested application :
Western Blot (WB), Immunocytochemistry (ICC)
app notes :
Immunocytochemistry: 1:1000. Western Blot: 1:1000. Optimal dilutions to be determined by researcher.
image1 heading :
Immunocytochemistry (ICC)
image2 heading :
Structure
other info1 :
Immunogen: Bovine brain tubulin expressed in HEK295 cell.
products categories :
Antibodies; Abs to Tubulin
products description :
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
ncbi gi num :
397913
ncbi acc num :
CAA80497.1
uniprot acc num :
P33625
ncbi mol weight :
49,892 Da
uniprot summary :
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Subunit structure: Dimer of alpha and beta chains. Subcellular location: Cytoplasm cytoskeleton. Post-translational modification: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively . By similarity.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling . By similarity. Sequence similarities: Belongs to the tubulin family.
size1 :
0.1 mg
price1 :
545 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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