protein

Activating Transcription Factor 3, Recombinant, Human, aa1-181 (ATF3, cAMP-dependent Transcription Factor ATF-3, Cyclic AMP-dependent Transcription Factor ATF-3)

MBS636121

0.1 mg

455 USD

Recombinant Protein

Activating Transcription Factor 3, Recombinant, Human, aa1-181 (ATF3, cAMP-dependent Transcription Factor ATF-3, Cyclic AMP-dependent Transcription Factor ATF-3)

Activating Transcription Factor 3, aa1-181

activating transcription factor 3; Cyclic AMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; activating transcription factor 3; Activating transcription factor 3

ATF3; ATF3; cAMP-dependent transcription factor ATF-3

ATF3_HUMAN

Recombinant corresponding to aa1-181 of human ATF3, expressed in E.coli. (NP_001665).

MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S

Highly Purified. >90% (SDS-PAGE)

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 2M Urea, 20% glycerol.

1mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Biology; MB-Transcription Factors

ATF3 (activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription, it is presumed, by sequestering inhibitory co-factors away from the promoter.

442422

AAA20506.1

P18847

~20.6kD

ATF-2 Transcription Factor Network Pathway (138006); Activation Of Genes By ATF4 Pathway (530772); Direct P53 Effectors Pathway (137939); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Id Signaling Pathway (198871); Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); PERK Regulated Gene Expression Pathway (105907); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963)

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5 -GTGACGT[AC][AG]-3 ), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor. Chromosomal Location of Human Ortholog: 1q32.3. Cellular Component: nucleoplasm; nucleolus; nucleus. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity. Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis

0.1 mg

455 USD