protein

Adiponectin, globular domain, Recombinant, Mouse, (His) (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)

MBS636045

0.05 mg

505 USD

Recombinant Protein

Adiponectin, globular domain, Recombinant, Mouse, (His) (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)

Adiponectin, globular domain,

adiponectin; Adiponectin; adiponectin; adipocyte-specific protein AdipoQ; adipocyte complement related protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ

Adipoq; Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30; Acdc; Acrp30; Apm1; ACRP30

ADIPO_MOUSE

E. Coli

The globular domain of mouse adiponectin (aa 104-247) is fused at the C-terminus to a His-tag.

Highly Purified. > or = 90% (SDS-PAGE)

Supplied as a liquid from a 0.2um-filtered solution in 30mM TRIS-Cl, pH 8.5.

1 mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months after receipt at -20 degre C. For Maximum recovery of product, centrifuge the oringal vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Biological Activity: Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.

Endotoxin: <1EU/ug (LAL)

Molecular Biology; MB-Adipocytes, Adipogenesis

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

87252711

NP_033735.3

NM_009605.4

Q60994

~17kD (SDS-PAGE)

Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198299); Developmental Biology Pathway (820229); Leptin And Adiponectin Pathway (198344); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862220); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway (522983); PPAR Signaling Pathway (83239); PPAR Signaling Pathway (694605)

adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma. Protein type: Secreted, signal peptide; Secreted; Hormone; Endoplasmic reticulum. Cellular Component: extracellular space; cell surface; collagen; protein complex; endoplasmic reticulum; extracellular region. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; hormone activity; sialic acid binding; receptor binding. Biological Process: negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; membrane hyperpolarization; positive regulation of cellular protein metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of heterotypic cell-cell adhesion; positive regulation of signal transduction; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; fatty acid beta-oxidation; positive regulation of fatty acid metabolic process; negative regulation of macrophage differentiation; negative regulation of low-density lipoprotein receptor biosynthetic process; cellular response to insulin stimulus; positive regulation of protein kinase activity; negative regulation of inflammatory response; brown fat cell differentiation; fatty acid oxidation; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

0.05 mg

505 USD