protein

Prolactin, Recombinant, Sheep (LTH, Luteotropic Hormone, Lactogenic Hormone)

MBS636015

0.01 mg

345 USD

Recombinant Protein

Prolactin, Recombinant, Sheep (LTH, Luteotropic Hormone, Lactogenic Hormone)

[Prolactin, Sheep]

[prolactin; Prolactin; prolactin]

[PRL; PRL; PRL]

PRL_SHEEP

E Coli

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys.

Highly Purified. >98% by SEC-HPLC and SDS-PAGE. Chromatographically purified.

Supplied as a lyophilized powder in 0.0045mM NaHCO3.

Lyophilized and reconstituted products are stable for 6 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Biological Activity: Ovine Prolactin is fully biologically active evidenced by inducing proliferation of Nb2 cells. Reconstitution: Reconstitute with sterile ddH2O to a concentration of >0.1mg/ml which can then be further diluted to other aqueous solutions.

Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Ovine Prolactin as a Reference Standard. Reconstitution: Reconstitute the lyophilized Ovine Prolactin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Molecular Biology; MB-Hormones, Steroids

Prolactin is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF. Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes. Recombinant Ovine Prolactin is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23kD.

57164329

NP_001009306.1

NM_001009306.1

P01240

25,778 Da

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family. Protein type: Secreted; Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide

0.01 mg

345 USD

0.05 mg

420