protein

CD45, Recombinant, Human (PTPRC, Protein Tyrosine Phosphatase, Receptor Type C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

MBS635951

0.05 mg

710 USD

Recombinant Protein

CD45, Recombinant, Human (PTPRC, Protein Tyrosine Phosphatase, Receptor Type C, B220, CD45, CD45R, GP180, LCA, L-CA, Leukocyte Common Antigen, LY5, T200)

[CD45]

[CD45; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200]

[PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45; L-CA]

PTPRC_HUMAN

NS0-derived human CD45

Gln-24 to Lys-575 of human CD45 (Accession #P08575) with a C-terminal 6-His tag. Applications:. Suitable for use in ELISA. Other applications not tested.

>90%

Supplied as a lyophilized powder from PBS, pH 7.3. Reconstitute with 500ul sterile PBS

Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA

Optimal dilutions to be determined by the researcher

Endotoxin: <0.1EU/ug. Activity: 1ug/ml of rhCD45 is captured onto a mouse anti-Polyhistidine antibody coated plate, the concentration of the rhGalectin 1 that produces 50% of the optimal binding is found to be ~0.35-1.4ug/ml

Molecular Biology; MB-CD Markers

CD45, previously called LCA (leukocyte common antigen) T200, or Ly5inmice, is member C of t he class 1 (receptor like) protein tyrosine phosphatase f amily (PTPRC). It is avaliably glycosylated 180-220kD transmembrane proteint hat is abundantly expressed on all nucleated cells of hematopoietic origin . CD45 has several isoforms, expressed according t o cell type, developmental stage and anti- genicexposure. The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes. The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronect in type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain t hat contains two hosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is t he shor test form, lackingexons 4,5 and 6 which encode aa32-191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved intoor out of the immunologicalsynapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily O glycosylated spliced regions and sialylation of N-linked carbohydrates. Within the I S, CD45 ephosphorylates and negatively regulates the Src family kinase, Lck. In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokne secretion and cell survival, partially overlapping in function with DEP-1/ CD148. CD45 deletion causes in severe i mmunodeficiency, while pont mutations may be associated with utoimmunedisorders.

44242008

AAS46946.1

P08575

61.6kD (predicted)

Adaptive Immune System Pathway (366160); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); EPO Receptor Signaling Pathway (198882); Fc Gamma R-mediated Phagocytosis Pathway (114228)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation. Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive

0.05 mg

710 USD