protein

Acetyl-Coa Carboxylase, Recombinant, Human (ACC1)

MBS635923

0.01 mg

780 USD

Recombinant Protein

Acetyl-Coa Carboxylase, Recombinant, Human (ACC1)

[Acetyl-Coa Carboxylase (ACC1)]

[acetyl-CoA-carboxylase; Acetyl-CoA carboxylase 1; acetyl-CoA carboxylase 1; ACC-alpha; acetyl-coenzyme A carboxylase alpha; acetyl-CoA carboxylase alpha<; ACC-alpha]

[ACC1]

[ACACA; ACACA; ACC1; ACCA; ACAC; ACCA; ACC1]

ACACA_BOVIN

> = 18%

Supplied as a liquid in 40mM Tris HCl, pH 8.0, 110mM Sodium chloride, 2.2mM KCl, 160mM Imidazole, 0.04% Tween20, 20% glycerol.

0.55 mg/ml

Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior for removing the cap. Shipping: Ships with Dry Ice.

Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Optimal dilutions to be determined by the researcher.

SDS-Page

Testing Data

Testing Data

Source: Recombinant human ACC1, aa1-2383, with C-terminal His tag, expressed in Baculovirus infected Sf9 cell expression system. Specific Activity: >=0.47pmol/min/ug. A 25ul ACC1 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM potassium citrate, 1mM DTT, 2mM sodium citrate, 100uM ATP, 12mM NaHCO3 and 20uM acetyl-Co-A at 30°C for 45 minutes. ATP reduction is detected using ADP-Glo Luminescent Kinase Assay Platform.

Molecular Biology; MB-Enzymes

6006405

CAB56826.1

Q9TTS3

270kD

Fatty Acid Biosynthesis Pathway (84179); Fatty Acid Biosynthesis Pathway (294); Insulin Signaling Pathway (84329); Insulin Signaling Pathway (501); Propanoate Metabolism Pathway (84245); Propanoate Metabolism Pathway (387); Pyruvate Metabolism Pathway (84241); Pyruvate Metabolism Pathway (373); Biotin-carboxyl Carrier Protein Assembly Pathway (123020); Fatty Acid Biosynthesis Initiation I Pathway (542829)

Function: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase . By similarity. Catalytic activity: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein]. Cofactor: Biotin . By similarity.Binds 2 manganese ions per subunit . By similarity. Enzyme regulation: By phosphorylation . By similarity. Activity is increased by oligomerization. Citrate and MID1IP1 promote oligomerization . By similarity. Pathway: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. Subunit structure: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity . By similarity. Subcellular location: Cytoplasm. Post-translational modification: Phosphorylation on Ser-1263 is required for interaction with BRCA1 . By similarity. Sequence similarities: Contains 1 ATP-grasp domain.Contains 1 biotin carboxylation domain.Contains 1 biotinyl-binding domain.Contains 1 carboxyltransferase domain.

0.01 mg

780 USD